4 x 400 Fresh-Soph
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| PL | TEAM | ATHLETES | TIME | TIME |
|---|---|---|---|---|
| 1 | Mainland | Marin Hawryluk, Taliyah Nixon, Autumn Demarest, Saraya Hinson | 5:08.70 | 4:54.00 |
| 2 | DeLand | Sydney Neira, Amy Nguyen, Sydney Bordeaux, Lilah Kersey | 6:26.33 | 5:04.19 |
| 3 | University (OC) | Lillie Pickens, Alea Smith, Shelsy Burgos Rosado, Sarahi Arroyo Canales | 5:03.80 | 5:33.84 |
| 4 | Lake Weir | 5:13.63 | 5:44.64 | |
| Father Lopez | Bianca Campos, Adelae Fulton, Marissa Stanberry, Frances Ginnochetti | DNS | DNS | |
| Olympia | Sandy Scott, Maddie Heald, Arlinda Roundtree, Korey Ardon | DNS | DNS |